Journal: Communications Biology

Article Title: Culture and gene expression profiling of Schwann cells from human sural nerve

doi: 10.1038/s42003-026-11023-z

Figure Lengend Snippet: A Phase-contrast images showing control human SC ( cSC ) and patient-derived SC ( pdSC ) cultures at DIV7. B Immunocytochemistry staining of SOX10 (magenta) and S100B (orange) in control human SC ( cSC ), patient-derived SC ( pdSC ) and fibroblast ( Fibroblast _1) cell cultures at DIV7. Nuclei were stained with DAPI (grey). Scale bar: 100 µm. C Gene expression of SOX10, S100B , and MBP in pdSCs ( n = 6), and cSCs ( n = 3). Median ± range. *, p < 0.05.

Article Snippet: Samples were then incubated with the primary antibodies monoclonal mouse to SOX10 (1:50, #SC-365692, Santa Cruz, RRID: AB_10844002), monoclonal rabbit to S100B (1:500, #ab52642, Abcam, RRID:AB_882426), monoclonal rat to MBP (1:100, #ab7349, Abcam, RRID:AB_305869) and/or monoclonal mouse to FN1 (1:100, #SC-8422, Santa Cruz, RRID: AB_627598) in 1% FBS/0.1% Triton/PBS over night at 4 °C.

Techniques: Phase Contrast, Control, Derivative Assay, Immunocytochemistry, Staining, Gene Expression

Journal: Development (Cambridge, England)

Article Title: Developmental dynamics of catshark cranial neural crest cells provide insights into gnathostome facial evolution

doi: 10.1242/dev.205258

Figure Lengend Snippet: 3D reconstruction of the catshark facial ectomesenchyme. (A,B) Lateral (A) and ventral (B) views of reconstructed CNCCs (St.18-19) and early facial ectomesenchyme (St.20-25). Only the frontonasal ectomesenchyme is shown in B. Arrowheads in B indicate the midfacial ectoderm. Dashed red and white lines indicate the positions of the tomographic slices shown in C and D. Scale bars: 500 µm. (C) Tomographic slices highlighting the position of CNCCs in the supraocular region at St.19 and facial ectomesenchyme at St.25. White arrowhead indicates a thin mesenchymal domain separating the ectoderm from the brain. Scale bars: 100 µm. (D) Tomographic slices highlighting the position of CNCCs and facial ectomesenchyme. White arrowhead indicates the first signs of a uniform frontonasal ectomesenchyme at St.25. Note the invagination of the olfactory placode divides the frontonasal ectomesenchyme into an anterior (AED) and posterior (PED) domain by St.25. Scale bars: 100 µm. (E) Genetic tracing of mouse CNCCs and their ectomesenchymal derivatives induced at E8.5 in Sox10-creERT2/R26Confetti embryos and analysed at E9.5 (left) and E10.5 (right). Embryos are shown in lateral and frontal views. Scale bars: 500 µm. White-outlined arrowheads indicate the midfacial ectoderm. AED, anterior ectomesenchymal domain; b, brain; e, eye; FN, frontonasal branch; FNM, frontonasal ectomesenchyme; h, heart; LNP, lateral nasal prominence; LpMes, lower periocular ectomesenchyme; Ma, mandibular branch; MdP, mandibular prominence; MNP, medial nasal prominence; MxP, maxillary prominence; og, optic groove; OlfP, olfactory placode; PED, posterior ectomesenchymal domain; Pha1-2, first and second pharyngeal arches; pMes, periocular ectomesenchyme; Tg, trigeminal CNCC; UpMes, upper periocular ectomesenchyme.

Article Snippet: The Sox10-CreERT2 line was coupled with the R26Confetti mouse strain (The Jackson Laboratory, code 013731) to generate Sox10-CreERT2/R26Confetti mice.

Techniques: Olfactory